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Production of Glycogen from Tahong (Perna viridis) Stock

Related Institution

Samar State University Mercedes Campus

Abstract

The focused of the study was to utilize Tahong (Perna viridis) specifically the liquid stock derived from steamed tahong in the production of glycogen. Glycogen is a polysaccharide chain of glucose units, a reserve carbohydrate in animals and a fast available energy supply. This study was the first of its kind since glycogen has been derived from a lot of different sources. Many processors in Jiabong and Villareal, Samar and even in household level throw away the stock derived from steam tahong since utilization of this waste product is not yet known. Series of trials were made to isolate glycogen from green mussel (Perna viridis). Glycogen has been isolated using 10% of acetic acid, treated with distilled water in boiling water bath for exactly 30 minutes at 80-100 degree Celsius. Glycogen was determined using qualitative colorimetric methods (Mendel et al, 1954). Successful extraction of glycogen was proved by acquiring positive results from Iodine reaction test and Benedict's tests. Iodine reaction test reacts with glycogen to form a brownish or reddish brown precipitate. Benedicts' reagent on the other hand reacts with glycogen to reduce a blue copper (II) ion to form a greenish solution. The average yield of semi-purified glycogen from shucked green bay mussel meat was about 35% while only 19% to that of liquid stock for every three (3) kilos of steamed tahong.

Objectives

1. To utilize tahong (Perna viridis) stock in the production of glycogen.

Specifically the project aimed to:

1. Design a process to extract glycogen from tahong stock
2. Evaluate the process in terms of
2.1 efficiency
2.2 quality of extracted glycogen


 
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